Ading the S Saturation recovery carried out by P450 Inhibitors reducing leads to a reduced sensitivity lower to its original level SSSS in FIG. 4B. An increase Increase the SSSS low signals observed pHER2 a consequence of the transition to S Saturation in S Saturation is not on the inhibition of signal pHER2. In addition to the Erh Increase the SSSS, we expect an increased sensitivity SSSS, the kinetic parameters i, k, and initial protein concentrations, EI. To study this effect, we calculated SSSS, i under various St Requirements of the STS, to induce the transition this. As you can see, the inhibition by pertuzumab increased pHER2 SSSS Ht sensitivity, i kinetic parameters of the reactions in PI3K/PTEN/AKT cycle and AKT phosphorylation. The significant erh Increase the sensitivity SSSS t, i k and EI occurswhen pertuzumab concentration IC 50 in its range and sensitivity, where SSSS pHER2 signal ht is obtained. A check in the SSSS sensitivity was observed, when i perform the sensitivity of the high throughput chemical screening transition strength in the way of PI3K/PTEN/AKT loss of PTEN by inhibiting PTEN activity T by BPV, where sensitivities are greatly reduced compared to their erh hte mirror on the action pertuzumab alone.
May be aware, this is exactly the opposite of the STS Bicalutamide Calutide parameter sensitivities by Change of PI3K and AKT activity t, according to PIK3CAmutations overexpression and AKT or achieved. Consequently, the sensitivity of t for the transition strength in the way PI3K/PTEN/AKT with the restoration of two signal S PACT saturation and low values of the sensitivities SSSS occurs, i kinetic parameters and the output values of the enzyme expression. Themechanism behind the decline of the SSSS, low γ I S is determined by the saturation of PIP3 production due to the imbalance of enzymatic activity in the th cycle PI3K/PTEN/AKT This imbalance leads to a restoration of the S saturation binding in the PIP3 response with ACT. Note that in the normal operation of the P concentration of PIP3 not saturated Is ttigt PIP2/PIP3 and the rest, as ordered by the cycle of PI3K / PTEN / AKT, the STS provides a regulatory mechanism. About the relationship between Ver Changes in the overall sensitivity and STS-cycle St Requirements of various PI3K/PTEN/AKT we calculated the sensitivity study are integrated with different levels of S and observed in the experimental inhibition by PACT, and combinations thereof, pertuzumabLY294002. PACT is by an increased inhibition Support hte sensitivity in the Hordenine presence of two or LY294002 or pertuzumabLY294002 pertuzumab compared with the control.
The resistance of the inhibition occurring PACT illustrated in connection with a decrease of S in the effect of the resistance, when combined with BPV pertuzumab. These membrane theoretical and experimental results show that the sensitivity of the STS for the inhibition of RTK, the kinetic parameters and levels of protein expression under the control PTEN/PI3K/AKT the cycle. ThusWe we have shown that increased Hte sensitivity to inhibitors SSN by an increased Hte sensitivity SSSS is supported, I the kinetic parameters of the proteins And their expression. This means that SN may be sensitive fragile compared to mutations, the kinetic properties of proteins and protein abundance to change. In particular, an increasing use sensitivitywould.