Because the ripples are all oriented perpendicular to the scratch

Because the ripples are all oriented perpendicular to the scratching direction, the sides of the Selinexor concentration obtained diamond dots are parallel to and with an angle of 135° to the horizontal line (highlighted by the white area

in Figure 4b). Finally, we used scratching angles of 0° and 45° (as shown in Figure 1e) to scratch the PC surface. Using a feed of 40 nm and normal load of 15.8 μN for a scratching of angle 0° and load of 14.8 μN for a scratching angle of 45°, we formed ripples with a period of 450 nm. The morphology and a FFT image of the fabricated surface are presented in Figure 4c. The length and shape of the dots are the same as the diamond-shaped nanodots above, except that the orientation of the dots has changed, with the sides perpendicular to and with an angle of 135° to the horizontal line (indicated by the white area in Figure 4c). Figure 4 Morphologies and 2D FFT images of 3D nanodot arrays. The scratching angles (a) 90° and 0°, Dactolisib supplier (b) 90° and 45°, (c) and 0° and 45° of the two-step scratching method. The above experimental results reveal that the length and orientation of nanodots can be regulated by manipulating the period of the ripples for a selected scratching direction. Using our two-step scratching method, by changing the period of the ripples formed using different scratching angles, complex, controllable 3D nanodot arrays can be fabricated easily.

Mechanism of ripples formation Anidulafungin (LY303366) As shown in Figure 5a,b, the process of ripple formation on PC sample surface can be presumed as an interaction of stick-slip [11] and crack formation [12] processes. When the tip scratches along the fast scanning direction,

the AFM tip indents the polymer surface and starts to push the surface material. In practice, the tip still sticks to the surface and is forced to hop over until the polymers that builds up in front of the tip offers enough resistance, so the bump is formed. Because the movement of the tip is a zigzag trace, the formed bump will be pushed forward and backward, and the rippling structures perpendicular to the scratching direction can be fabricated. For the typical ripple structures, the AFM morphology and modulus images are shown in Figure 5c,d. It can be found that the tip trace is clearly at the CHIR98014 supplier grooves but blurry at the ridges, which also confirmed that such ripples structures could be a stick-slip phenomenon. The cross-sections of the height and Young’s modulus of the ripples are shown in Figure 5e. The moduli are about 1.5 and 2.5 GPa at the ridges and grooves, respectively. For the raw PC surface, the modulus is about 2.45 GPa. The changing of the modulus may be a consequence of the crack existing within the bumps, which agrees well with the model that proposed by Dr. Khrushudov [12], as shown in Figure 5a. For the 3D nanodots arrays, the AFM morphology and modulus images are shown in Figure 5f,g.

Besides that, some authors had explored the potential association

Besides that, some authors had explored the potential association between the SULT1A1 polymorphism and breast cancer risk and it had also shown inconsistent results. Kotnis’ study showed that the polymorphism of SULT1A1 P505-15 ic50 Arg213His might predispose carriers to lung cancers, protect against colorectal cancers and increase the risk of breast cancer to Asian women but not the Caucasian women [11]. Recently Wang et al. meta-analyzed the relationships between SULT1A1 and breast cancer risk [12] and concluded

that there was no significant relationship between SULT1A1 R213 H polymorphism and the risk of breast cancer. However both meta-analysis were not perfect and may lead to underestimate this website the role of SULT1A1 polymorphism in breast carcinogenesis, because they did not include some eligible studies and neglected the valuable subgroup analysis such as menopausal status. It should be pointed out that there was new finding in results of the present study which was never founded in the previous. The

current meta-analysis approved to be a more precise estimation which included two more studies and a subgroup analysis according to menses status which came out statistical significance. Here we performed an updated meta-analysis which was specialized in breast cancer, including 16 studies with a subgroup analysis based on ethnicity and menopausal status, using Arg/Arg vs His/His, Arg/Arg vs Arg/His, dominant model (Arg/His+His/His vs Arg/Arg) and recessive model (His/His vs Arg/Arg+Arg/His). Methods Identification and analysis of relevant studies Two investigators (Yiwei Jang and Liheng Zhou) independently obtained relevant articles through searches of PubMed, EBSCO and Web of Science databases using the following words: ‘sulfotransferase or SULT’, ‘polymorphism’ and ‘breast cancer’. Studies had been case-control design and based on SULT1A1 Arg213His polymorphism either alone

or in combination with other genes Ibrutinib mouse and the language of publication was restricted to English. All of the studies required study design, publication, breast cancer cases, controls selection and genotyping methods. We excluded articles on only breast cancer patients or on healthy persons and one case-series study. In the end, 10362 breast cancer patients and 14250 controls from 16 case-control studies were selected for this meta-analysis. Data extraction The following data were collected from each included studies: first authors, year of publications, study population (categorized as Asian, Caucasian, African and others), sources of controls, menopausal status and the number of different genotype in all subjects.

Cascade, CO, USA) Incompatibility among primers was avoided by i

Cascade, CO, USA). Incompatibility among primers was avoided by in silico analysis of the formation of secondary structures, and oligonucleotides forming dimers with energy Selleck Dibutyryl-cAMP values lower than −6 kcal/mol and hairpins with Tm higher than 40C were discarded. The specificity of the oligonucleotides was first assessed by blastn (http://​www.​ncbi.​nlm.​nih.​gov/​blast/​Blast.​cgi?​PAGE=​Nucleotides). The reaction mix included 80 μg/tube of bovine serum albumin (Roche España, Madrid, Spain), 3.75 mM MgCl2 (Applied Biosystems), 200 μM dNTPs (Applied Biosystems) and 4U of AmpliTaq Gold® DNA Polymerase (Amersham Pharmacia Biotech, Cerdanyola del Vallès, Barcelona,

Spain). Primer concentrations ranged from 0.6 to 1 μM (Additional file 2: Table S2). The amplification cycles included an initial cycle of 94C for 9 min, followed by 40 cycles of 94C 30 s, 60C 1 min, and 72C 1 min, with a final extension at 72C for 10 min. The amplifications were performed in an MJ Research

Duvelisib PTC-200 (Bio-Rad Laboratories, S.A., Alcobendas, Madrid, Spain) in volumes of 50 μl. Hybridization by RLB was performed as described [25] using 48C for the hybridization and 40C for the conjugate and the washing steps. Concentration of probes ranged from 0.8 to 6.4 pmols/μl (Additional file 2: Table S2). Two overlapping films (SuperRX, Fujifilm España S.A., Barcelona, Spain), were used in each assay to obtain a less

and more exposed image for each membrane. Table 1 Scheme of the presence/absence of the Coxiella burnetii ORFs selected for the determination of genomic groups Target GGI GGII GGIII GGIV GGV GGVI GGVII GGVIII CBU0007 + + + − + + + + CBU 0071 + + + + − + + − CBU 0168 + + + − + + − + CBU 0598 + + − + + + + + CBU 0881 + + + + + − − − CBU 1805 + + + + − + + + CBU 2026 + − + + + + + + The sensitivity of the technique was checked with serial 10-fold dilutions of a purified DNA stock of the isolate Nine OSBPL9 Mile phase II (NMII) and the specificity was studied by subjecting to the method 104 genome equivalents of a selection of other bacterial species causing zoonoses or related illness (Orientia tsutsugamushi, Rickettsia conorii, R. typhi, Legionella pneumophila, Francisella tularensis subsp. holarctica, Bartonella henselae, Chlamydophila pneumoniae, and Mycoplasma pneumoniae). To assess the reproducibility of the methodology, DNA extracted from 2 different passages (n and n+10) of 5 reference isolates (NMI, CS-27, Priscilla, SQ217, F2) and a local isolate from cattle (273) (Additional file 1: Table S1) were analyzed. The results of the GT study were further analyzed by using InfoQuest™FP 4.50 (BioRad, Hercules, CA, USA). Clustering analyses used the binary coefficient (signaling pathway Jaccard) and UPGMA (Unweigthed Pair Group Method Using Arithmetic Averages) to infer the phylogenetic relationships.

The film morphology is obviously dependent on the oblique angle

The film morphology is obviously dependent on the oblique angle. For the film deposited at 0°, i.e., vertically deposited, a dense and flat surface was obtained as shown in Figure 1a. When the deposition angle was ≥60°, porous nanostructure was formed as shown in Figure 1b,c,d,e. It has been ��-Nicotinamide datasheet illustrated that during the OAD process, self-shadowing effect and limited surface diffusion lead to the formation of distinct columnar structure [11, 15]. With the deposition angle further increased to 85°, an aligned self-standing TiN nanorod arrays with length of ca. 270 nm and diameter of ca. 90 nm was obtained, which can be seen from the side view image in Figure 1f.

Figure 1 Top view SEM images of TiN films deposited at various oblique angles. (a) 0°, (b) 60°, (c) 70°, (d) 80°, (e) 85°, and (f) side view image Cediranib order of (e). Insets show the side view images. Figure 2 displays the XRD patterns of the TiN films deposited at various incident angles. It can be seen that the TiN film deposited at 0° exhibits (111) HM781-36B supplier and (200) diffraction of the face-centered cubic (FCC) structure of TiN (JCPDS 38–1420). The (111) peak becomes weaker for the films deposited at ≥60°, which can be attributed to the decrease in film thickness [16] and the formation of nanostructure during the OAD process. Figure 2 XRD patterns of the TiN film deposited at various incident angles. The

refractive index (n e) of the as-prepared TiN films was measured by spectroscopic ellipsometry Carbohydrate at wavelengths from 500 to 900 nm. Figure 3a plots the refractive index of the TiN film as a function of the wavelength. One can see that the film refractive index diminishes with the increase of the deposition angle. For a clear demonstration, we plot the variation of n e at 600 nm as a function

of the deposition angle, which is illustrated in Figure 3b. As the deposition angle increases from 0° to 85°, n e decreases from 2.15 to 1.68, which is the result of the formation of nanostructure [17]. For two non-absorbing components with volume fractions f i and refractive indices n i, the Bruggemann effective medium approximation gives [18] Figure 3 The refractive index spectra and refractive index at a wavelength of the TiN films. (a) The refractive index spectra of the TiN films in the wavelength range of 500 to 900 nm. (b) The refractive index at a wavelength of 600 nm and the calculated porosity of the films, as a function of the oblique angle. Herein, n e of a porous film is given by an average of air and material when the pore size is much smaller than the wavelength. Using the n e at 600 nm, the porosity of the above TiN films is calculated using the Bruggemann approximation, and the result is displayed in Figure 3b. When the deposition angle is increased, the porosity increases and reaches the maximum at the deposition angle of 85°, which is in accordance with that observed by SEM (see Figure 1).

Carvedilol produces dose-related improvements in left ventricular

Carvedilol produces dose-related improvements in left ventricular function and survival in subjects with chronic heart failure. MOCHA RSL3 cell line Investigators. Circulation. 1996;94:2807–16.PubMedCrossRef 8. Lowes BD, Gill EA, Abraham WT, Larrain JR, Robertson AD, Bristow MR, et al. Effects of carvedilol on left ventricular mass, chamber geometry, and mitral

regurgitation in chronic heart failure. Am J Cardiol. 1999;83:1201–5.PubMedCrossRef 9. Francis GS, Benedict C, Johnstone DE, Kirlin PC, Nicklas J, Liang CS, et al. Comparison of neuroendocrine activation in patients with left ventricular dysfunction with and without congestive Selleck Barasertib heart failure. A substudy of the Studies of Left Ventricular Dysfunction (SOLVD). Circulation. 1990;82:1724–9.PubMedCrossRef 10. Gilbert EM, Abraham WT, Olsen S, Hattler B, White M, Mealy P, et al. Comparative hemodynamic, left ventricular functional, and antiadrenergic effects of chronic treatment with metoprolol versus carvedilol in the failing heart. Circulation. 1996;94:2817–25.PubMedCrossRef 11. Morimoto S, Shimizu K, Yamada K, Hiramitsu S, Hishida H. Can beta-blocker therapy be withdrawn from patients with dilated cardiomyopathy? Am Heart J. 1999;138:456–9.PubMedCrossRef 12. Carson P, Ziesche S, Johnson G, Cohn

JN. Racial differences in response to therapy for heart failure: analysis of the vasodilator-heart failure trials. Vasodilator-Heart Failure Trial Study Group. J Card Fail. 1999;5:178–87.PubMedCrossRef PIK3C2G 13. Yancy CW. Heart failure in African Americans: a cardiovascular engima. J Card Fail. 2000;6:183–6.PubMedCrossRef 14. Thomas KL, East MA, Velazquez EJ, Tuttle RH, Shaw LK, O’Connor CM, et al. Outcomes by race and etiology of patients with left ventricular systolic dysfunction. Am J Cardiol. 2005;96:956–63.PubMedCrossRef 15. Liggett SB, Mialet-Perez J, Thaneemit-Chen S, Weber SA, Greene SM, Hodne D, et al. A polymorphism within a conserved beta(1)-adrenergic receptor motif alters cardiac function and beta-blocker response in human heart failure. Proc Natl Acad Sci USA. 2006;103:11288–93.PubMedCrossRef

16. Yancy CW, Fowler MB, Colucci WS, Gilbert EM, Bristow MR, Cohn JN, et al. Race and the response to adrenergic blockade with carvedilol in patients with chronic heart failure. N Engl J Med. 2001;344:1358–65.PubMedCrossRef 17. Packer M, Bristow MR, Cohn JN, Colucci WS, Fowler MB, Gilbert EM, et al. The effect of carvedilol on morbidity and mortality in patients with chronic heart failure. U.S. Carvedilol Heart Failure Study Group. N Engl J Med. 1996;334:1349–55.PubMedCrossRef 18. Packer M, Coats AJ, Fowler MB, Katus HA, Krum H, Mohacsi P, et al. Effect of carvedilol on survival in severe chronic heart failure. N Engl J Med. 2001;344:1651–8.PubMedCrossRef 19. Effect of metoprolol CR/XL in chronic heart failure. Metoprolol CR/XL Randomised Intervention Trial in Congestive Heart Failure (MERIT-HF). Lancet. 1999;353:2001–7.CrossRef 20.

As ARMS is very sensitive, routinely being able to detect at leas

As ARMS is very sensitive, routinely being able to detect at least 1% mutant in a background of normal DNA, selleck kinase inhibitor this may reduce the need for macro-dissection which eliminates a labour-intensive, time-consuming step in the analysis process. By coupling ARMS with real-time PCR product detection the analysis process is further shortened as PCR products

do not have to be processed, for example by agarose gel electrophoresis, and PCR product contamination is eliminated as reaction tubes do not need to be opened after the experiment is complete. As ARMS is sensitive it can also be used on samples where the tumour content is very low, for example circulating free (cf) tumour DNA shed from the tumour into the blood [19, 20] and in cytology samples [21, 22]. This can be an advantage when a tumour sample is not available, for example if the tumour is inoperable or so badly processed that no DNA is extractable. However, in our experience, the mutation detection rates using alternative sources of tumour such as cf DNA tend selleck to be lower than from a tumour biopsy. In this study we have evaluated ARMS and DNA sequencing only; however, there are a growing number of alternative methods being established that may merit evaluation. All methods have their own merits and are chosen according to the task e.g. clinical trial methodology may be different to those employed in the diagnostic setting for sensitivity, cost, availability and a variety of other reasons.

Test choice will differ as tests evolve and it is important to keep abreast of all available methods. In our experience, ARMS is more sensitive and robust at detecting defined somatic mutations

than DNA sequencing on clinical samples where the predominant sample type was FF-PET. Future developments in the field of mutation detection will be followed with anticipation as such technologies will be key to support personalised healthcare approaches that select patients for targeted treatments based on tumour mutation results. Acknowledgements We thank all the study investigators and patients involved in study D1532C00003 and the Iressa Survival Evaluation in Lung Cancer (ISEL) trial. Considerable thanks go to Brian Holloway Sulfite dehydrogenase (formerly of AstraZeneca) for his major contribution to the ISEL study and to John Morten (AstraZeneca) who contributed to the writing of the article. We thank Annette Smith, PhD, from Complete Medical Communications, who provided editing assistance funded by AstraZeneca. References 1. Schilsky RL: Personalized medicine in oncology: the future is now. Nat Rev Drug Discov 2010, 9: 363–366.PubMedCrossRef 2. Brambilla E, Gazdar A: Pathogenesis of lung cancer signalling pathways: roadmap for therapies. Eur Respir J 2009, 33: 1485–1497.PubMedCrossRef 3. Koshiba M, Ogawa K, Hamazaki S, Sugiyama T, Ogawa O, Kitajima T: The effect of formalin fixation on DNA and the extraction of high-molecular-weight DNA from fixed and embedded tissues. Pathol Res Pract 1993, 189: 66–72.PubMed 4.

Although phase 1 clinical trials have found that high doses (12 g

Although phase 1 clinical trials have found that high doses (12 g/day) of systemic CCM are safe [19], the use of polyphenols as antimicrobials is likely to

be limited to use as topical agents. The toxicity of EGCG was limited to minor skin irritation in mammalian models [20] at high concentrations and no adverse effects were seen with preparations containing up to 500 mg/Kg/day. phosphatase inhibitor In this study we present data on the activity of CCM alone and in combination with EGCG against a well characterised collection of MDR A. baumannii clinical isolates. Methods Chemicals reagents and media Curcumin powder (≥90% purity) extracted from Curcuma longa was purchased from the Cayman Chemical Company

(Michigan, USA). Epigallocatechin gallate (≥95% purity) was donated by Unilever PLC (Bedford, UK). All growth media (Iso-Sensitest broth) was purchased from Thermo Scientific (Basingstoke, UK), sterilised and made up locally according to the manufacturer’s instructions. Bacterial strains Nine Acinetobacter Ro-3306 clinical trial baumannii isolates were studied. These included the antibiotic susceptible type strain ATCC 19606 and 8 MDR clinical isolates. These have been extensively characterised previously and were chosen to be representative of UK epidemic clones (OXA-23 clones 1, 2, ‘Burn’) and/or exhibit resistance to colistin, tigecycline or produce metallo-β-lactamases (NDM enzymes) [21] Properties of the strains are detailed in Table 1. All isolates were stored at -70°C in microbank vials (Tucidinostat Thermofisher, UK) and thawed prior to their use. Table 1 Resistant determinants and sources of multidrug-resistant clinical isolates of Acinetobacter baumannii Isolate Properties Isolate source AB 19606 Antibiotic Susceptible type Strain. National Collection of type cultures AB 14 MDR PFGE defined UK OXA-23 clone 1 OXA-23-like carbapenemase producer. Dr J Turton, Public Health Tangeritin England, Colindale, UK AB 16 MDR PFGE defined

UK OXA-23 clone 2 OXA-23 carbapenemase producer. Dr J Turton, Public Health England, Colindale, UK AB 186 MDR PFEG defined UK ‘burn’ strain, OXA-23 producer. Dr J Turton, Public Health England, Colindale, UK AB 202 Tigecycline-resistant strain UK OXA-23 clone 1 isolate. Barts Health NHS Trust, London, UK AB 205 Colistin resistant UK OXA-23 clone 1 isolate. Barts Health NHS Trust, London, UK AB 292 MDR PFGE-defined OXA-23-like carbapenemase producer. Barts Health NHS Trust, London, UK AB 306 MDR NDM-1 carbapenemase producer. Barts Health NHS Trust, London, UK AB 308 MDR NDM-2 carbapenemase producer. S. Gottig, Goethe Universistat, Frankfurt, Germany Determination of minimum inhibitory concentrations Minimum inhibitory concentrations (MICs) were determined in corning 96-well microtitre plates (Corning, Amsterdam, The Netherlands).

Chemotherapy 2003,49(1–2):33–35 PubMed

Chemotherapy 2003,49(1–2):33–35.PubMed CBL0137 manufacturer 135. Marchetti F, Viale P: Current and future perspectives for levofloxacin in SIS3 mw severe Pseudomonas aeruginosa infections. J Chemother 2003,15(4):315–322.PubMed 136. Neu HC: Aztreonam activity, pharmacology, and clinical uses. Am J Med 1990,23;88(3C):2S-6S. 137. Malangoni MA: Aztreonam in treatment of intra-abdominal infections. Urology 1988,31(6 Suppl):28–32.PubMed 138. Bradford PA: Tigecycline: A first in class glycylcycline. Clin Microbiol Newsl 2004, 26:163–168. 139. Townsend ML, Pound MW, Drew RH: Tigecycline in the treatment of complicated intra-abdominal and complicated skin and skin structure

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Microbiol Infect Dis 2010,66(2):187–194.PubMed 142. Giamarellou H, Poulakou G: Multidrug-resistant Gram-negative infections: What are the treatment options? Drugs 2009,69(14):1879–1901.PubMed 143. Mezzatesta ML, Trovato G, Gona F, Nicolosi VM, Nicolosi D, Carattoli A, Fadda G, Nicoletti G, Stefani S: In vitro activity of tigecycline and comparators against carbapenem-susceptible and resistant Acinetobacter baumannii clinical isolates in Italy. Ann Clin Microbiol Antimicrob 2008, 7:4.PubMed 144. Curcio D, Fernandez F: Acinetobacter spp. susceptibility to

tigecycline: A worldwide perspective. J Antimicrob Chemother 2007, 60:449–450.PubMed 145. Scheetz MH, Qi C, Warren JR, Postelnick MJ, Zembower T, Obias A, Noskin GA: In vitro activities of various antimicrobials alone AMP deaminase and in combination with tigecycline against carbapenem-intermediate or-resistant Acinetobacter baumannii. Antimicrob Agents Chemother 2007, 51:1621–1626.PubMed 146. Bartlett JG, Gerding DN: Clinical recognition and diagnosis of Clostridium difficile infection. Clin Infect Dis 2008,46(Suppl 1):S12–8.PubMed 147. Nobre V, Harbarth S, Graf JD, Rohner P, Pugin J: Use of procalcitonin to shorten antibiotic treatment duration in septic patients. A randomized trial. Am J Respir Crit Care Med 2008, 177:498–505.PubMed 148. Hochreiter M, Köhler T, Schweiger AM, Keck FS, Bein B, von Spiegel T, Schroeder S: Procalcitonin to guide duration of antibiotic therapy in intensive care patients: A randomized prospective controlled trial. Crit Care 2009,13(3):R83.PubMed 149.

The present results are consistent with our previous research, de

The present results are consistent with our previous research, demonstrating that ND and microwave-radiofrequency carbon allotrope decreased the vascular selleck chemicals llc network in glioblastoma tumour and, consequently, their volume and weight. Moreover, diamond nanoparticles decreased the mRNA level of the main pro-angiogenic factors selleck compound VEGFA and bFGF [12]. ND also affected the transcription level of the human stress-responsive genes of cells exposed to stress (heat shock, cytotoxic and oxidative stress). It has been demonstrated that although ND did not show toxic effects on leukaemia cell line HL-60, it up-regulates the expression of the gene SOD1, responsible for the defence mechanism against

reactive oxygen species, and down-regulates the genes JUN, GADD45A and FRAP1, responsible for protection against genotoxic and cellular stress [22]. Moreover, the anti-angiogenic activity of nanoparticles has been related to their inhibitory effects on pro-angiogenic factors. Gold nanoparticles specifically

bind to VEGFA and bFGF and inhibit their interaction with cell membrane receptors [23, 24]. Among all the tested nanoparticles, only MWNT and more significantly ND showed anti-angiogenic activity. Nanomaterials with graphite structure (NG and GNS) did not alter blood vessel development. There are only a few studies on the biological activity of GNS. Wang et al. [25] showed that GNS oxide exhibited A-1210477 low toxicity in mice and human fibroblast cells. Furthermore, GNS displayed

low cytotoxicity in erythrocytes and fibroblasts [26], which together with our results suggests that GNS is highly biocompatible with the vascular system. Similarly, NG had no effect on CAM angiogenesis, although they have the same shape and similar size and are produced in the same way (but under different conditions) as ND [27], which had the strongest anti-angiogenic activity (Table 1). The strongest inhibition of vessel growth by ND may be linked to the inhibition of VEGF receptor (KDR) expression. VEGF is a major pro-angiogenic factor essential selleckchem for the development of the blood vessel network. It is controlled by the release of growth factors dependent on the oxygen level, with HIF-1 being one of the most important [3]. Hypoxia leads to the up-regulation of VEGF and, thus, the formation of new blood vessels, which consequently normalises the oxygen status. In tumours, high activity and fast divisions of tumour cells lead to oxygen deficiency that enhances vessel growth. KDR is also regulated by various signalling molecules in response to changes in oxygen concentration [28, 29]. Hypoxia leads to KDR up-regulation and activation of the angiogenic signalling cascade [30, 31]. Down-regulation of KDR by ND may decrease hypoxia-mediated angiogenesis and exert efficient and long-lasting anti-angiogenic effects. Moreover, chronic hypoxia can lead to further down-regulation of KDR [32].

Microbiology 2002,148(Pt 4):1027–1037 PubMed 63 Touati D: Iron a

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