Cellular imaging was carried out with a Nikon eclipse TE300 inverted fluorescent microscope (Nikon, Tokyo, Japan) (×200 magnification) equipped with a digital camera. Standard filters for DAPI (blue) or rhodamine (red) were used. The images were processed using the ImageJ program, applying the same setting parameters (brightness and contrast) to all samples, aiming to improve the blue and red fluorescence intensity. The overlap of the channels (red and blue) was EPZ015938 solubility dmso achieved using the BioImageXD program. Results Synthesis
of the product 1 The product 1 was obtained as a brilliant orange oily product after the reaction of the vegetable oil with rhodamine B in the presence of EDCI and DMAP (Figure 1) followed by purification through column chromatography. The TLC
image in Figure 2 shows spots of CAO (a), rhodamine B (b), the crude fluorescent product 1 (c), and Avapritinib chemical structure the purified fraction of the fluorescent product 1 (d) after revelation with UV light. As expected, the CAO spot was not revealed. Rhodamine B eluted with a retention factor (R f) of 0.14. Besides the characteristic spot of RhoB, several other spots can be observed for the elution of the crude product 1 (c). No spot presenting the R f of RhoB was observed for the purified product 1 (d). Figure 1 General reaction scheme. Rhodamine B coupling with hydroxyl click here group of ricinolein contained in the castor oil using DMAP and EDCI in dichloromethane to produce product 1. Figure 2 Thin layer chromatography (TLC) image. (A) Raw castor Dipeptidyl peptidase oil, (B) rhodamine B, (C) crude fluorescent product 1, and (D) purified fluorescent product 1. FTIR spectra of the starting raw materials of the reaction (CAO and RhoB), as well as of the purified fluorescent product 1, are shown in Figure 3. The product 1 (Figure 3 (A)) and CAO (Figure 3 (B)) showed similar FTIR spectra. However, in the FTIR spectrum for the product 1 (Figure 3 (A)), no band was observed at 1,595 cm-1 [C = O (carboxylic acid)] in contrast to the spectrum for the raw RhoB, in which this peak was present (Figure 3 (C)). Regarding the 1H-NMR spectrum, signals with a chemical shift
at low field (δ = 5.9 to 7) were observed only for the fluorescent product 1. Figure 3 Infrared spectra. (A) purified product 1 (product 1), (B) raw castor oil (CAO), and (C) rhodamine B (RhoB). The UV-vis spectrum for the purified product 1 showed λ max-ab at 519 nm. The spectrofluorimetry analysis was then performed using the above-mentioned wavelength for excitation of the samples. The emission spectrum for a sample containing 1.52 mg mL-1 of the fluorescent product 1 presented λ max-em at 567 nm with an intensity of 340 a.u. (Figure 4). Quantification of rhodamine B bound to the rhodamine-labeled triglyceride (product 1) was performed using the standard addition method (r > 0.99) indicating a concentration of bound dye of 0.517 ± 0.096 μmol per g of product 1. Figure 4 Fluorescence emission spectrum of the synthesized product 1 (1.52 mg mL -1 ).