The environmental conditions, pigment characteristics, growth activity etc., relating to the bloom are described in detail elsewhere ( Furuya et al.
2006). The primary Quizartinib objective of this work is to describe the phytoplanktonspecific absorption characteristics of the bay during the bloom. Secondly, an attempt is made to identify the pigments responsible for the major absorption peaks by resolving the overlapping features in the absorption spectra through derivative analysis. Samples were collected during fieldwork carried out in Manila Bay from 19 to 23 March 2004. The stations were distributed along two transects: an east-west (EW) transect between Manila and Limay (stn. MB7–13) and a north-south (NS) transect from the mouth of the bay to Pampanga (stn. MB1–5, MB10 & 11) (Figure 1). Physical parameters like temperature, salinity and conductivity were obtained using a portable CTD profiler. Samples for phytoplankton composition based on HPLC and phytoplankton this website spectral absorption were collected from different depths down to 23 m using a
Nansen sampler; surface (~ 5 cm) sampling was done using a bucket. Seawater samples (0.5–1 litres) were filtered onto 25 mm GF/F glass fibre filters under low vacuum pressure (< 25 hPa). The absorption spectra of total particulate matter was recorded in the wavelength range 350–750 nm at a resolution of 1 nm with a double-beam spectrophotometer (Shimadsu
MPS-2400) following the guidelines of Mitchell (1990). For each of the measured spectra, the optical density obtained at 750 nm was subtracted from all other wavelengths. The optical density of the total suspended matter was corrected for the path length amplification (β effect) according to Cleveland & Weidemann (1993). The optical density of detritus particles was measured following the pigment extraction method selleck chemicals llc of Kishino et al. (1985). The chlorophyll-specific absorption coefficients of phytoplankton (a*ph(λ)) were obtained by dividing the absorption coefficient of phytoplankton (aph(λ)) by the total Chl a (TChl a) concentration. TChl a and TChl b includes both mono and divinyl forms. Biomarker pigments were separated and quantified using reverse-phase gradient elution HPLC following Zapata et al. (2000). Seawater was filtered under a gentle vacuum (< 100 mm Hg) onto 25 mm glass fibre filters (Whatman GF/F) and stored immediately in liquid nitrogen. Pigments were extracted using methanol (95%), and the extract was mixed with 1 M ammonium acetate as the ion pairing reagent. It was then filtered through 0.2 μm PTFE filter (Whatman) and mixed with milli-Q water (5:1 v:v); thereafter 500 μl was injected into the HPLC system (Shimadzu) equipped with a Symmetry C8 column (Waters).