lamium. Methods: The plant
was dried and extracted by maceration in CH2Cl2: MeOH (1:1 v/v). Structures of the compounds from the CH2Cl2: MeOH (1:1) soluble fraction were determined by spectroscopic methods and compared with published data. The broth micro dilution method was used to evaluate the antimicrobial activities against bacteria and fungal species. Results: Four known compounds: aurantiamide acetate (1), lupeol (2), lespedin (3), sitosterol 3-O-β-D-glucopyranoside (4) and a mixture of sterols: campesterol (5), stigmasterol (6) and β-sitosterol (7) were isolated #moreover keyword# from CH2Cl2: MeOH (1:1) extract of B. lamium aerial parts. The crude extract, fractions and isolated compounds exhibited both antibacterial and antifungal activities that varied with microorganism (MIC=6.25
– 1000 µg/ml). Compound 3 was the most active (MIC=6.25 – 100 µg/ml) while Staphylococcus Inhibitors,research,lifescience,medical aureus, Enterococcus faecalis, Candida tropicalis and Cryptococcus neoformans were the most sensitive to all the tested compounds. Conclusion: The overall results of this study indicate that the CH2Cl2: MeOH (1:1) extract and some of isolated compounds have meantime interesting antimicrobial properties and can be used for the treatment of fungal and bacterial infections. Key Words: Antifungal, antibacterial, phytochemicals Introduction Inhibitors,research,lifescience,medical The emergence of human pathogenic microorganisms that are resistant to major classes of antibiotics has increased in recent years, due to the indiscriminate use of antimicrobial drugs.1 Inhibitors,research,lifescience,medical This has caused many clinical problems in the treatment of infectious diseases, and the antibiotics commonly used are sometimes associated with adverse effects such as hypersensitivity, allergic reaction and immunosuppression in Inhibitors,research,lifescience,medical the host.2 Thus, the search for the discovery of new antimicrobial agents is an urgent need. Cameroonian traditional medicine is increasingly solicited through tradipractitioners and herbalists in the treatments of infectious diseases. On
the other hand, about 80% of citizens in developing countries use traditional AV-951 medicine based on plant products.3 Traditionally, dry herbs are used either boiled in water like tea or as an infusion to treat systemic bacterial and fungal infections, or are directly applied on the skin or nails in a plaster form to treat local infections.4 Some species of the Acanthaceae family present antimicrobial activities.5 Previous chemical studies with species of this family were related to the isolation of alkaloids, iridoids, lignans, flavonoids, terpenoids and phenylpropanoids glycosides.6 Brillantaisia lamium (Nees) Benth is an erect branched herb from Acanthaceae family, which has a height of about and is found in moist tropical areas growing both in full sun and partial shade.