The tree was inferred from 1,470 aligned characters [10,11] of the 16S rRNA gene sequence under the maximum … Cells of the strain Yu37-1T are vibrio-shaped, http://www.selleckchem.com/products/crenolanib-cp-868596.html 0.4-0.5 x 1.4-2.0 ��m in size, occur singly or in pairs and stain Gram-negative  (Table 1 and Figure 2). No spore formation was detected for Yu37-1T . No data is available on the generation time of strain Yu37-1T. Nitrate is the only electron acceptor utilized, with ammonium as the end product . Elemental sulfur, sulfate, sulfite, nitrite, iron (III) oxide, manganese (IV) oxide, selenate, selenite, arsenate, arsenite, fumarate and oxygen are not used as alternative electron acceptors . Acetate, pyruvate, lactate, fumarate, succinate, malate, yeast extract, peptone and Casamino acids are utilized as electron donors with nitrate as the electron acceptor; fermentative growth has not been observed .
Strain Yu37-1T is strictly anaerobic and catalase negative . Table 1 Classification and general features of C. nitroreducens Yu37-1T according to the MIGS recommendations  Figure 2 Scanning electron micrograph of C. nitroreducens Yu37-1T Chemotaxonomy The predominant compounds in whole cell lipids of C. nitroreducens strain Yu37-1T are saturated branched-chain fatty acids: iso-C14:0 (26.3%), anteiso-C15:0 (24.1%), iso-C13:0 (7.7%), C18:0 (7.2%), C16:0 (6.2%), iso-C16:0 (5.7%) and anteiso-C13:0 (5.3%) . Menaquinone MK-8 was identified as the major quinone . Genome sequencing and annotation Genome project history This organism was selected for sequencing on the basis of its phylogenetic position , and is part of the Genomic Encyclopedia of Bacteria and Archaea project .
The genome project is deposited in the Genomes On Line Database  and the complete genome sequence is deposited in GenBank. Sequencing, finishing and annotation were performed by the DOE Joint Genome Institute (JGI). A summary of the project information is shown in Table 2. Table 2 Genome sequencing project information Growth conditions and DNA isolation C. nitroreducens Yu37-1T, DSM 19672, was grown anaerobically in DSMZ medium 1112 (Calditerrivibrio medium)  at 55��C. DNA was isolated from 0.5-1 g of cell paste using Qiagen Genomic 500 DNA Kit (Qiagen, Hilden, Germany) following the standard protocol as recommended by the manufacturer, with modification st/DL for cell lysis as described in .
DNA is available through the DNA Bank Network . Genome sequencing and assembly The genome was sequenced using a combination of Illumina and 454 sequencing platforms. All general aspects of library construction and sequencing can be found at the JGI website . Pyrosequencing reads were assembled using the Newbler assembler (Table 2). The initial Newbler assembly, consisting of seven contigs in four scaffolds, Dacomitinib was converted into a phrap assembly  by making fake reads from the consensus to collect the read pairs in the 454 paired end library.