E Windows program STATISTICA 6th Differences were considered ROCK Kinase significant at p0.05. RESULTS We loaded produced fluids and rigid liposomal MTO membrane, which additionally were Tzlich at its surface Surface with the sequence provided angiopeptide as targeting ligands for LRP to the transport through a dense barrier cell to investigate and determine the therapeutic potential of targeting these vesicles. Ligand-equipped liposomes prepared vesicles were prepared in a three-step process. Zun Highest rigid and fluid LUV were prepared by lipid film hydration and extrusion technology sp Ter, as described above. Membranfluidit t was of liposomes were characterized in a previous study by EPR measurements. Compared to rigid liposomes Lrigid, which were also included in the study for reasons of contr On the liposome membrane Lfluid a shorter period of relaxation in the N He the surface Surface and PLK also in the middle of the membrane.
This was also reflected in the control parameters for the three areas that could be distinguished. In all areas of the control parameter S was smaller than in liposomes in Lrigid JNK Signaling Pathway Lfluid. The n HIGHEST step was the encapsulation of the WTO technology for loading drugs away. These vesicles less than 200 nm, to reduce the absorption by the MPS and the size E is closely associated with a PI of less than 0.30, indicating that the homogeneous formulations were prepared. In the last step, LUV with trapped surfaces were MTO Surface by 3-derived peptide with 19 Ver Nderten or 1 Mi from PIT, as previously described. To determine the optimal conditions for the equipment of the liposomes with a ligand, experiments were performed using optimized fluorescence-labeled derivative of cholesterol, 3, designed to mimic a ligand molecule was. The molar Ratio, incubation temperature and incubation time were GE Changed. Optimal conditions for bind the maximum amount of ligand 3 were closing Written authority wrote to the derivative of Nelarabine cholesterol Angiopep first We decided to use of 2 mol% of the peptide, since incubation with an h Higher concentration of the ligand 3 is to improve the binding efficiency.
Figure 1 shows that the ligand of choice were obtained at 70, but we are watching the destruction Tion of the liposomes at this temperature. To avoid this, we have finally used an incubation temperature of only 28, and agrees on the incubation time to 12 hours to obtain a sufficient ligand binding. Flowering between k with about 84% of the ligand Nnte ultimately be preprared. This corresponds to about 17 mmol of ligand / mole of total lipids, derived from the results as obtained with the ligand model. We did not quantify this cholesterolconjugated for the original peptide sequence, since the absorption experiments showed that a sufficient adhesion liposomes carry ligands in our cell system. With the amount thisthe MTO in the brain was 0.020.03% and the maximum values were 5 min after injection with values of from about 1.5104 mg / g, 2.4104 mg / g and 3, the 4104 mg / g for free MTO , L4fluid, L4fluid and LG, which indicates a clear hierarchy of the free drug, closing of drugs for liposomal drugs Lich followed by transports equipped ligand liposomes. Liposomes and therapeutic effect Lrigid Lfluid with and without ligands were closing Stating their therapeutic effect in human breast cancer cells tested MK.