Considering that males have larger muscle fibers, we expected to see higher mRNA levels of genes involved in muscle protein biosynthesis in the male and or higher expression levels of www.selleckchem.com/products/Enzastaurin.html genes involved in protein catabolic processes in the female. Instead, our investigation revealed that the genes involved in transcription and post transcriptional RNA processing, ribosome con struction and mRNA translation were consistently expressed at higher levels in female biceps. This obser vation suggests that females, at least at the transcrip tional level, have a greater potential for protein biosynthesis Inhibitors,Modulators,Libraries due to a higher efficiency of gene transcrip tion and translation machinery. Indeed, a recent study reported that women had higher rates of whole body protein turnover and skeletal muscle protein synthesis than men at both young and old age.

The higher protein turnover rates may underlie the need for increased levels of translational machinery. In comparison to females, males in the rested state demonstrated higher expression levels of genes which enriched GO terms relevant to protein modification by small protein removal. The key genes driving the enrich ment of these terms included several ubiquitin specific Inhibitors,Modulators,Libraries peptidases. Since ubiquitin conjugation for targeted protein degradation via the proteasome system plays a crucial role in muscle protein proteolysis, it is rea sonable to postulate that ubiquitin removal AV-951 might outweigh ubiquitination in male muscle thus aiding in muscle protein preservation.

However, it should be pointed Inhibitors,Modulators,Libraries out that the Inhibitors,Modulators,Libraries enrichment of deubiqui tination relevant GO terms and KEGG pathways in male muscle may be simply a result of including the Y chromosome in the analysis considering the most signif icant gene, ubiquitin specific peptidase 9, is Y linked. Since the biological function of USP9Y in ske letal muscle is still unclear, we can only speculate that interference of deubiquitination factors in the protein catabolic process might play a role in protein accumula tion in males. Sex alters the time course of gene transcriptional regulation Crizotinib ALK after RE In the present study, we observed that RE induced an extensive alteration in skeletal muscle transcriptome throughout the 24 h recovery period in both male and female muscles. When examining the GO terms and KEGG pathways enriched with differentially expressed genes across the four conditions, we recog nized a striking difference between the sexes in the time course of RE induced transcriptome alteration. Male muscles responded to the exercise stimuli with pro longed alterations in transcript contents, where most of the GO terms and KEGG pathways that were signifi cantly enriched at 4 h post exercise also remained significant at 24 h post exercise.