CrossRef 22. Thompson JD, Higgins DG, Gibson TJ: CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequencing weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res 1994, 22:4673–4680.PubMedCrossRef 23. Bryson K, McGuffin LJ, Marsden RL, Ward JJ, Sodhi JS, Jones DT: Protein structure prediction servers at University College London.

Nucleic Acids Res 2005,33(Web Server):W36-W38.PubMedCrossRef 24. Kelley LA, Sternberg MJE: Protein structure prediction on the web: a case study using the Phyre server. Nat Protoc 2009, 4:363–371.PubMedCrossRef 25. Zhang S, Flores-Cruz Z, Zhou L, Kang BH, Fleites L, Gooch MD, Wulff NA, Davis MJ, Duan Y, Gabriel BAY 11-7082 price DW: ‘ Ca . Liberibacter asiaticus’ carries an excision plasmid prophage and a click here chromosomally integrated prophage that becomes lytic in plant infections. Mol Plant-Microbe Interact 2011, 24:458–468.PubMedCrossRef 26. Gmitter FG, Hu X: The possible role of Yunnan, China, in the origin of contemporary citrus species (Rutaceae). Econ Bot 1990, 44:267–277.CrossRef

27. CAL101 Ayalewa S, Blackwood ER, Confer AW: Sequence diversity of the immunogenic outer membrane lipoprotein PlpE from Mannheimia haemolytica serotypes 1, 2, and 6. Vet Microbiol 2006, 114:260–268.CrossRef 28. Belland RJ, Morrison SG, Carlson JH, Hogan DM: Promoter strength influences phase variation of neisserial opa genes. Mol Microbiol 1997, 23:123–135.PubMedCrossRef Authors’ contributions XW, CZ and JC participated in the design of the study. Cediranib (AZD2171) XW carried out laboratory work and sequence analysis and drafted the manuscript. CZ helped to draft the manuscript. XD maintained the strain collection and edited the manuscript. HS was responsible for strain collection and participated in PCR and sequence alignment. JC performed the statistical analysis, drafted and edited the manuscript. All authors read and approved the final manuscript.”
“Background The production of virulence factors in Staphylococcus aureus is coordinated by a network of two-component systems, global regulators and transcription factors, allowing optimal

adaptation of the pathogen to a changing environment and stress conditions encountered during the various stages of infection. A central regulatory element of virulence factor production in S. aureus is the accessory gene regulator agr, a two-component quorum sensor regulating gene expression in a growth-dependent manner. The main effector molecule of the agr operon is the regulatory RNAIII [1], which is responsible essentially for the upregulation of secreted proteins in the post-exponential phase. RNAIII transcription is enhanced by the staphylococcal accessory regulator SarA [2] and reduced by the alternative sigma factor σB in strain Newman [3, 4]. SarA is a winged helix transcription factor influencing many virulence genes [5, 6].