While in the acquisition session, gerbils had been positioned for min habituation while in the chamber and then a shock was delivered each time the animal crossed from a single plate to an alternative. Each and every animal obtained ten shocks, each separated from your upcoming by a minimum of s devoid of shock. Subsequently, the animal was placed individually inside a cage with sawdust floor and totally free access to foods and water for h. Medication or automobile had been administered with both automobile or fluoxetine min before the retest session. Animals had been placed again in the four plate chamber for min as well as the duration of foot tapping recorded Forced swim check in mice As previously , male CD mice were placed in individual glass cylinders containing cm of water at . C for min. Duration of immobility was measured more than the last min.
Drug or motor vehicle were administered min prior testing Marble burying behaviour in mice As previously , min soon after drug or motor vehicle administration, mice had been individually placed a cool way to improve in transparent polycarbonate cages containing cm of sawdust and glass marbles evenly spaced against the wall. Thirty min later on, the quantity of marbles buried no less than two thirds was recorded Social interaction in gerbils The method was as previously . Gerbils have been positioned in excess weight matched pairs in opposite corners of the extremely illuminated open topped arena to get a min session. Each gerbils acquired precisely the same treatment method min ahead of testing. Data analysed had been duration of active social interaction, i.e. time spent grooming, following, sniffing, biting, jumping or crawling above or beneath the other animal Vogel conflict test in rats Research have been undertaken as previously . The grid and spout of a water bottle were linked to an Anxiometer that recorded licks and delivered shocks.
Throughout the days preceding testing, rats have been limited to h every day tap water . On day , just just after water delivery they were isolated in cages having a grid floor. On day , rats have been placed from the check cage by using a grid floor and the session initiated following the animal had manufactured licks and acquired a first, mild shock through the spout. Thereafter, a shock was delivered full report each and every th lick for min. The quantity of licks emitted throughout the min session was recorded. Control animals not receiving shocks had been made use of to assess free of charge drinking. Drugs or motor vehicle had been given i.p min prior to testing Influence on locomotor activity in gerbils, mice and rats As previously , the influence of medication was evaluated working with transparent polycarbonate cages equipped with two infrared beams cm over the floor and cm apart and white Plexiglas cages equipped with two rows of four photocells cm above the floor and cm apart for mice.
Cages were linked by an interface to a microcomputer . Animals acquired drug or car have been permitted min for adaptation to chambers, then locomotion was recorded over min or min .