Furthermore, polymorphisms in the human IL-4 gene associated with reduced IL-4 production are significantly linked with increased S. aureus colonization (Emonts et al., 2008). These data are consistent with the TH2 anti-inflammatory fibrotic response as being critical for controlling S. aureus infection. Whether this is directly because of the Selleckchem LY2835219 induction of polyamine synthesis has yet to be reported, but the acquisition of speG-encoding ACME would

counter increased spermine levels in fibrotic tissue perhaps explaining the association of USA300 CA-MRSA with severe skin/soft tissue infections. How do we reconcile a significant role for SpeG in S. aureus pathogenesis with the lack of a strong ACME phenotype in most model infections (Diep et al., 2008a; Montgomery et al., 2009)? One explanation could be that the observed increase in α-hemolysin and Protein A expression upon ACME inactivation in USA300 could overcompensate for the resulting polyamine sensitivity (Diep et al., 2008a). Another Sirolimus purchase possibility is that the Arc operon on ACME actually drives excess polyamine production necessitating SpeG-mediated spermine detoxification.

The Arc operon consists of genes that convert l-arginine to l-ornithine and CO2 while producing ATP and ammonia. The resulting l-ornithine is exchanged for extracellular l-arginine by the l-arginine/l-ornithine antiporter ArcD effectively converting extracellular l-arginine to l-ornithine. Thus, the Arc operon could skew the flux of host l-arginine away from iNOS toward polyamine

synthesis rendering speG essential (Fig. 2). Deleting all of ACME might allow the host to partition available l-arginine toward NO-production, an immune effector that S. aureus is known to effectively resist (Richardson et al., 2006, 2008; Hochgrafe et al., 2008). This is consistent with the presence of speG on ACME islands that harbor the auxiliary arc gene cluster (Fig. 2). While this hypothesis could explain the modularity of ACME that results in ∆speG attenuation, it has several aspects that require experimental attention. First, all strains of S. aureus already encode an Arc operon on the core chromosome that could also result in excess host polyamine synthesis, yet SpeG is only associated with ACME-positive USA300 S. aureus. This could be explained by the fact that Thalidomide the chromosomal Arc operon is only expressed under conditions of low oxygen and low glucose and little is known about ACME Arc expression in S. aureus (Makhlin et al., 2007). Second, a dominant MRSA clone of ST22 lineage in Irish hospitals harbors an ACME island with an arc gene cluster but appears to lack a speG homologue (Shore et al., 2011). Another issue is that significant CA-MRSA disease in Latin America is caused by USA300 clones that lack ACME (Reyes et al., 2009). Thus, ACME may contribute to colonization and virulence, but it cannot fully explain the predominance of USA300 in CA-MRSA disease in North America.