R inhibitors. TKI i-Kit and Src tyrosine kinase inhibits c. Apatinib was used in a Phase III clinical trial in China for its efficacy in the treatment of gastric cancer, lung cancer, small cell, not to determine. No studies PCI-34051 HDAC Inhibitors of the effect apatinib in cell lines or animal models that overexpress ABCB1 or ABCG2 transporter studied. Therefore, to determine be carried out experiments in this study whether apatinib, the effectiveness of the Herk Potentiate mmlichen antineoplastic drugs via interaction with ABC transporters in MDR cancer cells. Materials and Methods Apatinib reagents were from Jiangsu Hengrui Medicine Co., having a molecular structure as shown in Fig. S1A. Monoclonal Body against ABCB1 and ABCC1 were from Santa Cruz Biotechnology.
ABCG2 Antique Get body from Chemicon International, Inc. Antique act Body a Mi et al. Page 2 Cancer Res Author manuscript, increases available in PMC 15th AZD6244 MEK inhibitor October 2011. PA Author Manuscript NIH-PA Author Manuscript NIH-PA Author Manuscript NIH-produced antique Body monoclonal Cell Signaling Technology, Inc. 219 C, and 34 were purchased from Signet Laboratories Inc. BXP. Phosphorylated Akt, phosphorylated extracellular Re signalregulated kinase, MAPK1 / 2, and glyceraldehyde-3-phosphate dehydrogenase Antique Body were purchased from Kangchenjunga Co.. Iodoarylazidoprazosin was obtained from Perkin Elmer Life Sciences. Dulbecco, modified Eagle’s medium and RPMI 1640 were products of Gibco BRL. Rhodamine 123, 1 3, 5 diphenylformazan, paclitaxel, doxorubicin, vincristine, verapamil, topotecan and other chemicals were from Sigma Chemical Co.
. Cell Lines The following cell lines were cultured in DMEM or RPMI 1640 ergs with 10% FBS at 37 complements cultured in a humidified atmosphere re of 5% CO 2: Lines of human breast cancer cells MCF-7, the DOX selected COOLED derivative ABCB1-overexpressing MCF 7 / adr resistant ABCG2-overexpressing MCF andflavopiridol 7/FLV1000 lines were kindly provided by Dr. Bates SE. The human oral carcinomas Epidemo Was obtained from KB cell line and its derivative overexpressing ABCB1 VCR selected hlt KBv200 as a gift from Dr. Yi Liu Xu, H Pital cancer program in Beijing. KB 3 1 and KB/ABCC1 transfected cells were kindly provided by Dr. S. Akiyama available. The following cell lines were obtained from Dr.
Bates SE: People of c S1-lon cell line and mitoxantrone selected hlt derivative ABCG2-overexpressing S1 M1 80 acquired mutations in the specificity of t MXR / BCRP / ABCP gene in Ver modify substrate overexpressing cells MXR / BCRP / ABCP, Cancer Res 61, 6635 6639, prime re HEK293 human embryonic kidney cell line PcDNA3.1 its gene, ABCB1, ABCC1 and ABCG2 stable lines of transfected cells HEK293/pcDNA3.1 HEK293/ABCB1 and multidrug resistance-associated protein transport mediated by an oral inhibitor, CBT ® first Biochem. Pharmacol 75: 1302 1312, 2008, and R2 HEK293/ABCG2 HEK293/ABCC1. Biochemistry 41: 10123 10 132, 2002. All transfected cells were cultured in medium containing 2 mg / ml G418. All resistant cells were obtained by comparing the resistance of both parental cells more sensitive to medication and checked expression of ABC transporters authenticated. All cells were developed in a culture medium without drugs for 2 weeks prior to the assay. Athymic mice Nacktm animals, 5 g of 6 weeks and weighing from 18 24, were obtained from the Center for Experimental Anime