The presence of ectomycorrhizae increased net Pi uptake rates into intact Pinus pinaster roots at low or high soil P levels. The expression patterns of HcPT1 and HcPT2 indicate that the two fungal phosphate transporters
may be involved in uptake of phosphate from the soil solution under the two soil P availability conditions used.”
“The Wilson-Cleary health outcomes model is a hypothesized pathway linking traditional clinical variables to health-related quality of life (HRQL). This study tested the LCL161 mouse application of the Wilson-Cleary model to a patient population with generalized anxiety disorder (GAD) using longitudinal clinical trial data.
These secondary analyses pooled data from three similar 8-week, placebo-controlled, double-blind, randomized, multicenter trials of quetiapine XR therapy in GAD.
Relevant health assessments for the model concepts included the Clinical Global Impression-Severity of Illness, Hamilton Rating Scale for Anxiety, the Pittsburgh Sleep Quality Index and Quality of Life Enjoyment and Satisfaction Questionnaire-Short JIB-04 datasheet Form. A lagged path model tested whether the hypothesized relationships at baseline and week 8 between the concepts of the adapted Wilson-Cleary model were consistent with the observed data.
The resulting model fit the data (RMSEA = 0.077; CFI = 0.98; NFI = 0.96) and explained 56% of the variance in overall quality of life assessment at baseline and 69% of the variance in this assessment at week 8. Moderate to strong relationships between the adjacent hypothesized concepts support the specified model.
This adapted Wilson-Cleary model for health outcomes CRT0066101 order validated in GAD should improve the understanding and usefulness of health status measurements in this condition and increase the applications of this model to other clinical trial data.”
“A sensitive and selective liquid chromatography mass spectrometry (LC-MS) method for determination of imatinib in rat plasma was developed. After addition of metoprolol as internal standard (IS), protein precipitation
by acetonitrile was used as sample preparation. Chromatographic separation was achieved on a Zorbax SB-C18 (2.1 mm x 150 mm, 5 pm) column with acetonitrile-0.1% formic acid as mobile phase with gradient elution. Electrospray ionization (ESI) source was applied and operated in positive ion mode; selective ion monitoring (STM) mode was used to quantification using target fragment ions m/z 494 for imatinib and m/z 268 for the IS. Calibration plots were linear over the range of 10-2000 ng/mL for imatinib in plasma. Lower limit of quantification (LLOQ) for Imatinib was 10 ng/mL. Mean recovery of imatinib from plasma was in the range 803-96.6%. CV of intra-day and inter-day precision were both less than 9%. This method is simple and sensitive enough to be used in pharmacokinetic research for determination of imatinib in rat plasma.