With Bst DNA polymerase, the selleckchem target DNA can be clearly amplified for 60 min at 64 degrees C in a simple water
bath. The detection sensitivity of the LAMP assay for the detection of V. alginolyticus is about 3 center dot 7 x 102 CFU ml-1 (3 center dot 7 CFU per reaction). LAMP products could be judged with agar gel or naked eye after the addition of SYBR Green I. There were no cross-reactions with other bacterial strains indicating a high specificity of the LAMP. The LAMP method was applied to detect V. alginolyticus-infected fish tissues effectively.
Conclusions:
The LAMP established in this study is a simple, sensitive, specific, inexpensive and rapid protocol for the detection of V. alginolyticus.
Significance and Impact of the Study:
This LAMP method provides an important diagnostic tool for the detection of V. alginolyticus infection both in the laboratory and field.”
“Aims:
To test the efficacy of acceptable photoantimicrobial agents against bacterial pathogens
implicated in complicated urinary tract infection (UTI) in comparison with conventionally employed antibacterials.
Methods and Results:
Toluidine blue (TB), methylene blue (MB), 5-aminolaevulinic acid (ALA), trimethoprim and levofloxacin were employed in the study against the typical UTI-implicated pathogens Staphylococcus aureus, Klebsiella pneumoniae, Escherichia coli, Pseudomonas aeruginosa, Enterococcus Veliparib ic50 faecalis and Proteus mirabilis. Standard bacterial cell culture was used to assay the activity both in the dark and under 660-nm LED-illuminated conditions.
TB and MB were highly photoactive across the range and exhibited rapid kill rates, their effects being assayed after 20-min illumination, rather VX770 than the 18-h incubation employed with the other compounds. Trimethoprim was inactive against all bacteria except Pr. mirabilis, while levofloxacin maintained highly bactericidal activity throughout. ALA required high concentrations for effective action but, for porphyrin production in situ, also required an 18-h incubation.
Conclusions:
TB
and MB were highly and rapidly photobactericidal in comparison with the remaining agents tested.
Significance and Impact of the Study:
Ubiquitous catheterization of geriatric patients offers a portal for light delivery to the urinary tract. The photoantimicrobial approach thus offers considerable potential.”
“Aims:
The aim of this study was to develop a real-time quantitative PCR test to recognize and quantify the DNA levels of the increasingly important barley pathogen Ramularia collo-cygni.
Methods and Results:
The method described uses specifically designed primers and a molecular beacon probe based on an internal transcribed spacer (ITS) sequence. Pathogen extracted from barley leaves could be quantified to the picogram level in both leaves showing symptoms of infection and symptomless barley leaves.
Conclusions:
A relationship between R.