PubMedCrossRef 11. Nuanualsuwan S, Cliver DO: Pretreatment to avoid positive selleck RT-PCR results with inactivated viruses. J Virol Methods 2002, 104:217–225.PubMedCrossRef 12. Topping JR, Schnerr H, Haines J, Scott M, Carter

MJ, Willcocks MM, Bellamy K, Brown DW, Gray JJ, Gallimore CI, Knight AI: Temperature inactivation of Feline calicivirus vaccine strain FCV F-9 in comparison with human noroviruses using an RNA exposure assay and reverse transcribed quantitative real-time polymerase chain reaction-A novel method for predicting virus infectivity. J Virol Methods 2009, 156:89–95.PubMedCrossRef 13. Fittipaldi M, Nocker A, Codony F: Progress in understanding preferential detection of live cells using viability dyes in combination with DNA amplification. J Microbiol Methods 2012, 91:276–289.PubMedCrossRef 14. Fujimoto J, Tanigawa K, Kudo Y, Makino H, Watanabe K: Identification and quantification of viable Bifidobacterium breve strain Yakult in human faeces by using strain-specific primers and propidium monoazide. J Appl Microbiol 2011, 110:209–217.PubMedCrossRef 15. Josefsen MH, Löfström C, Hansen TB, Christensen LS, Olsen

JE, Hoorfar J: Rapid quantification of viable Campylobacter bacteria on chicken carcasses, using KU-60019 real-time PCR and propidium monoazide treatment, as a tool for quantitative risk assessment. Appl Environ Microbiol 2010, 76:5097–5104.PubMedCrossRef 16. Nocker A, Camper AK: Novel approaches toward preferential detection of viable cells using nucleic acid amplification techniques. FEMS Microbiol Lett 2009, 291:137–142.PubMedCrossRef 17. Yáñez MA, Nocker A, Soria-Soria E, Múrtula R, Martínez L, Catalán V:

Quantification of viable Legionella pneumophila cells using propidium monoazide combined with quantitative PCR. J Microbiol Methods 2011, 85:124–130.PubMedCrossRef 18. Nocker A, Cheung CY, Camper AK: Comparison of propidium monoazide with ethidium monoazide for differentiation of live vs. dead bacteria by selective removal of DNA from dead cells. J Microbiol Methods 2006, 67:310–320.PubMedCrossRef 19. Kim K, Katayama H, selleck products Kitajima M, Tohya Y, Ohgaki S: Development of a real-time RT-PCR Atorvastatin assay combined with ethidium monoazide treatment for RNA viruses and its application to detect viral RNA after heat exposure. Water Sci Technol 2011, 63:502–507.PubMedCrossRef 20. Kim SY, Ko G: Using propidium monoazide to distinguish between viable and nonviable bacteria, MS2 and murine norovirus. Lett Appl Microbiol 2012, 55:182–188.PubMedCrossRef 21. Parshionikar S, Laseke I, Fout GS: Use of propidium monoazide in reverse transcriptase PCR to distinguish between infectious and noninfectious enteric viruses in water samples. Appl Environ Microbiol 2010, 76:4318–4326.PubMedCrossRef 22. Graiver DA, Saunders SE, Topliff CL, Kelling CL, Bartelt-Hunt SL: Ethidium monoazide does not inhibit RT-PCR amplification of nonviable avian influenza RNA. J Virol Methods 2010, 164:51–54.PubMedCrossRef 23.