(XLS 192 KB) Additional file 2: Table

S2. The table shows the primers sequences used in this study. (XLS 34 KB) Additional file 3: Table S4. The table details the calculation of the Discriminatory Index for each typing methods including IS900 RFLP, MIRU-VNTR and PFGE (SnaB1, Spe1) used alone and in combination. The table details the calculation of the allelic diversity (h) at a locus MIRU-VNTR using Nei’s index. (XLS 84 KB) Additional file 4: Figure S1. The figure shows the new IS900 RFLP PD0332991 profiles obtained from analysis selleck chemical with strains S of subtype III. (PPT 156 KB) Additional file 5: Table S3. The table describes the MIRU-VNTR allelic distribution among the strains of Map of type S and C and other Mac members. (DOCX 26 KB) References 1. Alexander DC, Turenne CY, Behr MA: Insertion and deletion events that define the

pathogen mycobacterium avium subsp. Paratuberculosis. J Bacteriol 2009,19(3):1018–1025.CrossRef 2. Collins DM, Gabric DM, de Lisle GW: Identification of two groups of mycobacterium paratuberculosis strains by restriction endonuclease analysis and DNA hybridization. J Clin Microbiol 1990,28(7):1591–1596.PubMed 3. De Lisle GW, Collins DM, Huchzermeyer HF: Characterization of ovine strains of mycobacterium paratuberculosis by restriction endonuclease analysis and DNA hybridization. Onderstepoort J Vet Res 1992,59(2):163–165.PubMed 4. Janagama HK, Kumar S, Bannantine JP, Kugadas A, Jagtap P, Higgins L, Witthuhn B, Sreevatsan S: Iron-sparing response of mycobacterium avium subsp. Paratuberculosis is strain dependent. BMC Microbiol 2010,10(1):268.PubMedCrossRef 5. Janagama HK, Jeong K, Kapur V, Coussens P, Sreevatsan Z-IETD-FMK clinical trial S: Cytokine responses of bovine macrophages to diverse clinical mycobacterium avium subspecies paratuberculosis strains. BMC Microbiol 2006, 6:10.PubMedCrossRef 6. Motiwala AS,

Janagama HK, Paustian ML, Zhu X, Bannantine JP, Kapur V, Sreevatsan S: Comparative transcriptional analysis of human macrophages exposed to animal and human isolates of mycobacterium avium subspecies paratuberculosis with diverse genotypes. Infect Immun 2006,74(11):6046–6056.PubMedCrossRef 7. de Juan L, Alvarez unless J, Aranaz A, Rodriguez A, Romero B, Bezos J, Mateos A, Dominguez L: Molecular epidemiology of types I/III strains of mycobacterium avium subspecies paratuberculosis isolated from goats and cattle. Vet Microbiol 2006,115(1–3):102–110.PubMedCrossRef 8. Stevenson K, Hughes VM, de Juan L, Inglis NF, Wright F, Sharp JM: Molecular characterization of pigmented and nonpigmented isolates of mycobacterium avium subsp. Paratuberculosis. J Clin Microbiol 2002,40(5):1798–1804.PubMedCrossRef 9. Supply P, Magdalena J, Himpens S, Locht C: Identification of novel intergenic repetitive units in a mycobacterial two-component system operon. Mol Microbiol 1997, 26:991–1003.PubMedCrossRef 10. Supply P, Mazars E, Lesjean S, Vincent V, Gicquel B, Locht C: Variable human minisatellite-like regions in the mycobacterium tuberculosis genome.