The product was repeatedly washed OSI906 with methanol and separated with a strong NdFeB permanent magnet. The obtained
powder was identified as magnetite by XRD. Dimension of the core/shell structure not exceeding 5 nm and their spherical shape were confirmed by TEM analysis. The FT-IR analysis identified the organic coating agent, i.e., lauric acid on the surface of the magnetite nanoparticles. In order to fabricate a modified surface of prosthetic device, core/shell/EO nanofluid was used to create a coated shell. The layer of core/shell/EO nanofluid on the prosthetic device was achieved by submerging the catheter pieces in 5 mL of nanofluid (represented by solubilized core/shell/EO in CHCl3 (0.33% w/v) aligned in a magnetic field of 100 kgf applied for 1 s. The catheter pieces were allowed to dry at room temperature. The see more rapid drying was facilitated by the convenient GS-1101 cell line volatility of chloroform . The coated prosthetic devices were then sterilized by ultraviolet irradiation for 15 min. Figure 1 presents a schematic representation of biofilm development on the surface of the prosthetic
device coated/uncoated with anti-pathogenic nanofluid. Figure 1 Biofilm development on the surface of the prosthetic device coated/uncoated with anti-pathogenic nanofluid. (a)staphylococcal biofilm development on the surface of the prosthetic device, (b) nano-modified surface of the prosthetic device, (c) inhibition of staphylococcal biofilm development on the nano-modified surface of the prosthetic device. TG analysis The thermogravimetric (TG) analysis of [email protected] and [email protected]@EO was followed with a Netzsch TG 449C STA Jupiter instrument (Netzsch, Selb, Germany). Samples were PAK5 screened with 200 mesh prior to analysis, placed in an alumina crucible, and heated at 10 K·min−1 from room temperature to 800°C, under the flow of
20 mL min−1of dried synthetic air (80% N2 and 20% O2). Biofilm development on nano-modified prosthetic device surface The adherence of S. aureus ATCC 25923 was investigated in six multiwell plates using a static model for monospecific biofilm developing. Catheter pieces of 1 cm with and without coated shell were distributed in plastic wells (one per well) and immersed in the liquid culture medium represented by nutrient broth. The plastic wells were inoculated with 300 μL of 0.5 McFarland microbial suspensions and incubated for 24 h at 37°C. After incubation the culture medium was removed, and the prosthetic device samples were washed three times in phosphate buffered saline (PBS) in order to remove the nonadherent strains and moved into sterile wells. Then, fresh broth was added, the incubation being continued for 72 h.