Umazenil, acetate NGmonomethyl arginine and L 4,5-dihydro-2 4,4,5,5 tetramethyl 1H imidazolyl an oxy-3-oxide, a potassium salt, and 1H oxadiazoloquinoxalin 1-ci. Flumazenil was suspended in 0.3% Tween 80. ODQ was dissolved in dimethyl sulfoxide St and added with distilled water to reach a final concentration of DMSO of 1%. The NMMA and Carboxy PTIO was bacteriostatic saline Gel solution St. Based on previous experience in our laboratory, was L-NMMA dose of 10 mg / kg dose of carboxy PTIO was 0.6 mg / kg dose of ODQ was 0.01 mg / kg, and the dose of flumazenil was 10 mg / kg . The effect of drug pretreatment on the time spent alone in the light compartment of the box You light / dark is 2 in Figure In antagonismexperiments the drugs were administered ip prior to treatment to different groups of M Nozzles 30 min after the end of 60 min and 30 min before the test HBO2 treatment anxiolytic. This time interval was calculated as the midpoint of the anxiolytic response to HBO2. The statistical analysis results are displayed as meanS.D. Differences between means were analyzed by one-way ANOVA and post hoc Bonferroni test. The results of controlled mouse In section 84.69.6 s spent in light Maraviroc Selzentry compartment of the box You light / dark. Increased at 60 min HBO2 treatment at 2.5, 3.0 or 3.5 Ht ATA the amount of time spent on the room. The most robust anxiolytic effect occurred after treatment with HBO2 at 3.0 ATA. HBO2 treatment increased Ht fa A lot of time spent in the compartment lamp 127.712.3, 133.211.2 and 119.38.9 s 30, 60 and 90 min after cessation of treatment. Separate groups of mice M, 60 min at 3.0 ATA HBO 2 treatment undergone, but again U different pretreatment drugs 30 min after HBO 2 treatment.
Usually 60 minutes after treatment HBO2, spent Mice an average 133.211.2 s in the light compartment of the box You light / dark. Groups of mice from M With L NMMA, carboxy PTIO, ODQ and flumazenil last 77.314.8, 96.29.7 s, s and s, 99.8 11.6 99.514.5 pretreated in the light chamber. Reducing the time spent in light compartment was statistically significant for all four drug pretreatments. Different cars for each pretreatment drug alone produces no significant effect on the time spent in light compartment. It should ALK also be noted that, Mice treated with L NMMA alone 81.810.1 s spent in the lamp compartment, spent Mice with carboxy PTIO alone 84.710.0 s discussed in the light compartment, Mice treated with ODQ alone spent 130 911 .2 s in the lamp compartment, and M mice treated with flumazenil alone spent 89.49.2 s in the light compartment. Only the effect of ODQ was significantly regulated differently On. Research focus more NO in the tt angstl do Send effects of these drugs. Prevented the anxiolytic effect of chlordiazepoxide as the mouse was elevated plus maze by pretreatment with systemic nitric oxide synthase L-arginine NGnitro, this inhibitory effect of L NOARG was stereospecifically YOUR BIDDING by intracerebroventrikul Re administration of inverted L-Arginine but not D . The anxiolytic effects such as nitric oxide also by pretreatment Ren with a plurality of NOS inhibitors or antisense oligodeoxynucleotide against st.